Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-28723 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28723, RRID:AB_1913927
- Product name
- Rabbit Polyclonal NCAPH2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCAPH2 Antibody [NBP1-28723] - Detection of Human CAP-H2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-CAP-H2 antibody NBP1-28723 used for WB at 1 mcg/ml (A and B) and used for IP at 10 mcg/mg lysate. CAP-H2 was also immunoprecipitated by rabbit anti-CAP-H2 antibody NBP1-28682, which recognizes a downstream epitope. For blotting immunoprecipitated CapH2, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCAPH2 Antibody [NBP1-28723] - Detection of Human CapH2 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from HeLa and (50 ug) from 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CapH2 antibody NBP1-28723 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NCAPH2 Antibody [NBP1-28723] - Detection of human CapH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CapH2 antibody NBP1-28723 used for IP at 6 ug per reaction. CapH2 was also immunoprecipitated by rabbit anti-CapH2 antibody NBP1-28682. For blotting immunoprecipitated CapH2, NBP1-28723 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: NCAPH2 Antibody [NBP1-28723] - FFPE section of human skin carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NCAPH2 Antibody [NBP1-28723] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CAP-H2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining using Immunohistochemistry Accessory Kit.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: NCAPH2 Antibody [NBP1-28723] - Detection of human CAP-H2 by immunohistochemistry. Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CAP-H2 (NBP1-28723). Detection: DAB