AF7385

BSCL2 antibody from R&D Systems

GNG3LG, seipin, SPG17

Western blot

Supportive data in Antibodypedia

Immunocytochemistry

Supportive data in Antibodypedia

Provider product page for AF7385

Antibody Data

Product number

AF7385

Provider

R&D Systems

Product name

Human Seipin/BSCL2 Antibody

Provider product page

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Seipin/BSCL2 in direct ELISAs and Western blots.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

Q96G97


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Seipin/BSCL2 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Seipin/BSCL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7385) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Seipin/BSCL2 at approximately 70 and 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Seipin/BSCL2 in Human Mesenchymal Stem Cells. Seipin/BSCL2 was detected in immersion fixed human mesenchymal stem cells differentiated into adipocytes using Sheep Anti-Human Seipin/BSCL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7385) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.