HPA028748

antibody from Atlas Antibodies

Targeting: SLC12A3 NCCT

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA028748

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA028748, RRID:AB_10603886

Product name

Anti-SLC12A3

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human SLC12A3, Gene description: solute carrier family 12 (sodium/chloride transporter), member 3, Alternative Gene Names: NCCT, Validated applications: IHC, Uniprot ID: P55017, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Human Nephrogenesis can Persist Beyond 40 Postnatal Days in Preterm Infants

Carpenter J, Yarlagadda S, VandenHeuvel K, Ding L, Schuh M
Kidney International Reports 2024;9(2):436-450

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Klotho is highly expressed in the chief sites of regulated potassium secretion, and it is stimulated by potassium intake

Jung H, Pham T, Su X, Grigore T, Hoenderop J, Olauson H, Wall S, Ellison D, Welling P, Al-Qusairi L
Scientific Reports 2024;14(1)

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Chung E, Deacon P, Hu Y, Lim H, Park J
2024

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Lineage Tracing and Single-Nucleus Multiomics Reveal Novel Features of Adaptive and Maladaptive Repair after Acute Kidney Injury

Gerhardt L, Koppitch K, van Gestel J, Guo J, Cho S, Wu H, Kirita Y, Humphreys B, McMahon A
Journal of the American Society of Nephrology 2023;34(4):554-571

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Bmp7 drives proximal tubule expansion and determines nephron number in the developing kidney

Taglienti M, Graf D, Schumacher V, Kreidberg J
Development 2022;149(14)

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Long-term Clinical Course after Living Kidney Donation by a Patient with Gitelman Syndrome Harboring a Compound Heterozygous Mutation of the SLC12A3 Gene.

Kamejima S, Yamamoto I, Tajiri A, Tanno Y, Ohkido I, Yokoo T
Internal medicine (Tokyo, Japan) 2021 May 15;60(10):1567-1572

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In Situ Classification of Cell Types in Human Kidney Tissue Using 3D Nuclear Staining

Woloshuk A, Khochare S, Almulhim A, McNutt A, Dean D, Barwinska D, Ferkowicz M, Eadon M, Kelly K, Dunn K, Hasan M, El‐Achkar T, Winfree S
Cytometry Part A 2020;99(7):707-721

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Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney

Marable S, Chung E, Park J
Journal of the American Society of Nephrology 2020;31(11):2543-2558

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β-catenin regulates the formation of multiple nephron segments in the mouse kidney

Deacon P, Concodora C, Chung E, Park J
Scientific Reports 2019;9(1)

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Monkeys mutant for PKD1 recapitulate human autosomal dominant polycystic kidney disease

Tsukiyama T, Kobayashi K, Nakaya M, Iwatani C, Seita Y, Tsuchiya H, Matsushita J, Kitajima K, Kawamoto I, Nakagawa T, Fukuda K, Iwakiri T, Izumi H, Itagaki I, Kume S, Maegawa H, Nishinakamura R, Nishio S, Nakamura S, Kawauchi A, Ema M
Nature Communications 2019;10(1)

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Reduced Abd-B Hox function during kidney development results in lineage infidelity

Magella B, Mahoney R, Adam M, Potter S
Developmental Biology 2018;438(2):84-93

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H+-ATPase B1 subunit localizes to thick ascending limb and distal convoluted tubule of rodent and human kidney

Frische S, Chambrey R, Trepiccione F, Zamani R, Marcussen N, Alexander R, Skjødt K, Svenningsen P, Dimke H
American Journal of Physiology-Renal Physiology 2018;315(3):F429-F444

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Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome

Marable S, Chung E, Adam M, Potter S, Park J
JCI Insight 2018;3(14)

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Conserved and Divergent Molecular and Anatomic Features of Human and Mouse Nephron Patterning

Lindström N, Tran T, Guo J, Rutledge E, Parvez R, Thornton M, Grubbs B, McMahon J, McMahon A
Journal of the American Society of Nephrology 2018;29(3):825-840

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EED, a member of the polycomb group, is required for nephron differentiation and the maintenance of nephron progenitor cells

Zhang L, Ettou S, Khalid M, Taglienti M, Jain D, Jung Y, Seager C, Liu Y, Ng K, Park P, Kreidberg J
Development 2018;145(14)

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Affinity Proteomics Reveals Elevated Muscle Proteins in Plasma of Children with Cerebral Malaria

Kim K, Bachmann J, Burté F, Pramana S, Conte I, Brown B, Orimadegun A, Ajetunmobi W, Afolabi N, Akinkunmi F, Omokhodion S, Akinbami F, Shokunbi W, Kampf C, Pawitan Y, Uhlén M, Sodeinde O, Schwenk J, Wahlgren M, Fernandez-Reyes D, Nilsson P
PLoS Pathogens 2014;10(4):e1004038

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human kidney and liver tissues using HPA028748 antibody. Corresponding SLC12A3 RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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