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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-34947 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DCTD Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, PC-3, U87-MG, SK-N-SH, human DCTD-transfected 293T cells. Predicted reactivity: Mouse (94%), Rat (94%), Zebrafish (90%), Xenopus laevis (90%), Chicken (90%), Rhesus Monkey (97%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DCTD Polyclonal Antibody detects DCTD protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human DCTD-transfected 293T cells. 12% SDS PAGE. DCTD Polyclonal Antibody (Product # PA5-34947) dilution: 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DCTD Polyclonal Antibody detects DCTD protein by Western blot analysis. A. 30 µg NT2D1 whole cell lysate/extract. B. 30 µg PC-3 whole cell lysate/extract. C. 30 µg U87-MG whole cell lysate/extract. D. 30 µg SK-N-SH whole cell lysate/extract. 12% SDS PAGE. DCTD Polyclonal Antibody (Product # PA5-34947) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DCTD Polyclonal Antibody detects DCTD protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. DCTD Polyclonal Antibody (Product # PA5-34947) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
