H00001635-M01

antibody from Invitrogen Antibodies

Targeting: DCTD

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001635-M01

Provider

Invitrogen Antibodies

Product name

DCTD Monoclonal Antibody (4B9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: RTAENKLDTK YPYVCHAELN AIMNKNSTDV KGCSMYVALF PCNECAKLII QAGIKEVIFM SDKYHDSDEA TAARLLFNMA GVTFRKFIPK CSKIVIDFDS INSRPSQKLQ

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4B9

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DCTD over-expressed 293 cell line, cotransfected with DCTD Validated Chimera RNAi (Cat # H00001635-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DCTD monoclonal antibody (M01), clone 4B9 (Cat # H00001635-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DCTD over-expressed 293 cell line, cotransfected with DCTD Validated Chimera RNAi (Cat # H00001635-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DCTD monoclonal antibody (M01), clone 4B9 (Cat # H00001635-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of DCTD expression in transfected 293T cell line by DCTD monoclonal antibody (M01), clone 4B9.Lane 1: DCTD transfected lysate(20.016 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DCTD is 0.03 ng/mL as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to DCTD on formalin-fixed paraffin-embedded human placenta. Antibody concentration is 3 µg/mL.