PA5-36012

antibody from Invitrogen Antibodies

Targeting: ZNF257 BMZF-4

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36012

Provider

Invitrogen Antibodies

Product name

ZNF257 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.67 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Integrative analysis of DNA methylation and gene expression identified cervical cancer-specific diagnostic biomarkers.

Xu W, Xu M, Wang L, Zhou W, Xiang R, Shi Y, Zhang Y, Piao Y
Signal transduction and targeted therapy 2019;4:55

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ZNF257 Polyclonal Antibody detects ZNF257 protein at nucleus by immunofluorescent analysis. Sample: HL-60 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZNF257 protein stained by ZNF257 Polyclonal Antibody (Product # PA5-36012) diluted at 1:500. Blue: Hoechst 33342 staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 GABRA2, SLC5A8, and ZNF257 are weakly expressed in human cervical squamous cell carcinoma (CSCC). IHC staining of the indicated proteins in a human CSCC tissue array containing 93 intact cancer tissues and paired normal adjacent tissues. Representative images are shown in the left panels. Magnified images are shown in red boxes. The H -score-based quantification results are shown in the right panels. ** p < 0.01, *** p < 0.001, Student's t test