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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA1-1960 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMB9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-1960 detects proteasome 20S LMP2 from human and mouse cells.
- Concentration
- 1 mg/mL
Submitted references Evaluation of Immunoproteasome-Specific Proteolytic Activity Using Fluorogenic Peptide Substrates.
Interferon β induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice.
Inhibition of apoptosis in acute promyelocytic leukemia cells leads to increases in levels of oxidized protein and LMP2 immunoproteasome.
Neuronal induction of the immunoproteasome in Huntington's disease.
Kim S, Park SH, Choi WH, Lee MJ
Immune network 2022 Jun;22(3):e28
Immune network 2022 Jun;22(3):e28
Interferon β induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice.
Chort A, Alves S, Marinello M, Dufresnois B, Dornbierer JG, Tesson C, Latouche M, Baker DP, Barkats M, El Hachimi KH, Ruberg M, Janer A, Stevanin G, Brice A, Sittler A
Brain : a journal of neurology 2013 Jun;136(Pt 6):1732-45
Brain : a journal of neurology 2013 Jun;136(Pt 6):1732-45
Inhibition of apoptosis in acute promyelocytic leukemia cells leads to increases in levels of oxidized protein and LMP2 immunoproteasome.
Khan MA, Oubrahim H, Stadtman ER
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 10;101(32):11560-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 10;101(32):11560-5
Neuronal induction of the immunoproteasome in Huntington's disease.
Díaz-Hernández M, Hernández F, Martín-Aparicio E, Gómez-Ramos P, Morán MA, Castaño JG, Ferrer I, Avila J, Lucas JJ
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Dec 17;23(37):11653-61
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Dec 17;23(37):11653-61
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of proteasome 20S LMP2 in 50 µg mouse embryo fibroblast cell extract using Product # PA1-1960.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µglysate) of HeLa (Lane 1), HeLa treated with IFN gamma (100 ng/mL IFN gamma for 72h) (Lane 2), THP-1 (Lane 3), THP-1 treated with PMA (50 ng PMA for 48h) and LPS (200 ng/mL LPS for 24 h) (Lane 4), U-937 (Lane 5), Raji (Lane 6) and Ramos (Lane 7).The blots were probed with PSMB9 Rabbit polyclonal Antibody (Product # PA1-1960, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 22 kDa band corresponding to PSMB9 was observed across the cell line tested. Apart from the desired band a non-specific band was also observed in untreated cells. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of PSMB9 was done on SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with PSMB9 Rabbit Polyclonal Antibody (PA11960, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control..
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
