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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- HPA051857 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA051857, RRID:AB_2681641
- Product name
- Anti-ZNF217
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ZNF217, Gene description: zinc finger protein 217, Alternative Gene Names: ZABC1, Validated applications: ICC, Uniprot ID: O75362, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references The PCOS GWAS Candidate Gene ZNF217 Influences Theca Cell Expression of DENND1A.V2, CYP17A1, and Androgen Production.
Stiff stroma increases breast cancer risk by inducing the oncogene ZNF217
The dark side of ZNF217, a key regulator of tumorigenesis with powerful biomarker value
Waterbury JS, Teves ME, Gaynor A, Han AX, Mavodza G, Newell J, Strauss JF 3rd, McAllister JM
Journal of the Endocrine Society 2022 Jul 1;6(7):bvac078
Journal of the Endocrine Society 2022 Jul 1;6(7):bvac078
Stiff stroma increases breast cancer risk by inducing the oncogene ZNF217
Northey J, Barrett A, Acerbi I, Hayward M, Talamantes S, Dean I, Mouw J, Ponik S, Lakins J, Huang P, Wu J, Shi Q, Samson S, Keely P, Mukhtar R, Liphardt J, Shepherd J, Hwang E, Chen Y, Hansen K, Littlepage L, Weaver V
Journal of Clinical Investigation 2020;130(11):5721-5737
Journal of Clinical Investigation 2020;130(11):5721-5737
The dark side of ZNF217, a key regulator of tumorigenesis with powerful biomarker value
Cohen P, Donini C, Nguyen N, Lincet H, Vendrell J
Oncotarget 2015;6(39):41566-41581
Oncotarget 2015;6(39):41566-41581
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Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting ZNF217 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting ZNF217 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:197
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
