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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA044700 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA044700, RRID:AB_2679052
- Product name
- Anti-SPDL1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human SPDL1, Gene description: spindle apparatus coiled-coil protein 1, Alternative Gene Names: CCDC99, FLJ20364, hSpindly, Validated applications: IHC, Uniprot ID: Q96EA4, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma
Expression of spindle assembly checkpoint proteins BubR1 and Mad2 expression as potential biomarkers of malignant transformation of oral leukoplakia: an observational cohort study
Delgado L, Monteiro L, Silva P, Bousbaa H, Garcez F, Silva J, Brilhante-Simões P, Pires I, Prada J
Animals 2022;12(22):3082
Animals 2022;12(22):3082
Expression of spindle assembly checkpoint proteins BubR1 and Mad2 expression as potential biomarkers of malignant transformation of oral leukoplakia: an observational cohort study
Monteiro L, Silva P, Delgado L, Amaral B, Garcês F, Salazar F, Pacheco J, Lopes C, Bousbaa H, Warnakulasuriya S
Medicina Oral Patología Oral y Cirugia Bucal 2021
Medicina Oral Patología Oral y Cirugia Bucal 2021
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Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting SPDL1 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting SPDL1 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:233
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human testis and liver tissues using HPA044700 antibody. Corresponding SPDL1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
