PA1-12382

antibody from Invitrogen Antibodies

Targeting: PPP1CC PP1C, PP1gamma

Immunoprecipitation

Antibody data

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Validation data

PPP1CC protein structure - PA1-12382 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of PPP1CC was achieved by transfecting HeLa with PPP1CC specific siRNAs (Silencer® select Product # s719). Western blot analysis (Fig. a) was performed using membrane enriched extracts from the PPP1CC knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PP1 gamma Polyclonal Antibody (Product # PA1-12382, 1:1000 dilution) and Rabbit anti-Sheep IgG (H+L), Secondary Antibody, HRP (Product # 61-8620, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PPP1CC.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-PP1 gamma Polyclonal Antibody (Product # PA1-12382) and a 37 kDa band corresponding to PPP1CC was observed across cell lines and tissues tested. Membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), NTERA-2 (Lane 2), HEK-293 (Lane 3), Hep G2 (Lane 4), tissue extracts of Mouse lung (Lane 5), Mouse uterus (Lane 6) and Rat eye (Lane 7)were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Rabbit anti-Sheep IgG (H+L), Secondary Antibody, HRP (Product # 61-8620, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).