MA5-17250

antibody from Invitrogen Antibodies

Targeting: PPP1CC PP1C, PP1gamma

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

MA5-17250

Provider

Invitrogen Antibodies

Product name

Anti-PP1 gamma Monoclonal Antibody (AF11F10)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is MOLT-4 cells.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

AF11F10

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PP1G using anti-PP1G monoclonal antibody (Product # MA5-17250) on (Lane 1) HeLa Cell lysate, (Lane 2) 293T Cell lysate, (Lane 3) Jurket Cell lysate, (Lane 4) K562 Cell lysate, (Lane 5) SK-N-MC Cell lysate, (Lane 6) RD Cell lysate and (Lane 7) IMR32 Cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of PPP1CC was achieved by transfecting HeLa with PPP1CC specific (Silencer® select Product # s719). Western blot analysis (Fig. a) was performed using Modified whole cell extracts (1% SDS) from the untransfected cells (Lane 1), non-specific scrambled siRNA transfected cells (Lane 2) and PPP1CC knockdown cells (Lane 3). The blot was probed with PP1 gamma Monoclonal Antibody (AF11F10) (Product # PA5-17250, 0.1 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to PPP1CC.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using PP1 gamma Monoclonal Antibody (AF11F10) (Product # MA5-17250) and a 36 kDa band corresponding to PPP1CC was observed across the cell lines and tissue tested. Whole cell Extracts of (30 µg lysate) of HeLa (Lane 1),NTERA-2 (Lane 2), HEK 293(Lane 3) and mouse lung (Lane 4 were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (0.1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).