Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- MA5-17250 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-PP1 gamma Monoclonal Antibody (AF11F10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- A suggested positive control for this product is MOLT-4 cells.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AF11F10
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PP1G using anti-PP1G monoclonal antibody (Product # MA5-17250) on (Lane 1) HeLa Cell lysate, (Lane 2) 293T Cell lysate, (Lane 3) Jurket Cell lysate, (Lane 4) K562 Cell lysate, (Lane 5) SK-N-MC Cell lysate, (Lane 6) RD Cell lysate and (Lane 7) IMR32 Cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of PPP1CC was achieved by transfecting HeLa with PPP1CC specific (Silencer® select Product # s719). Western blot analysis (Fig. a) was performed using Modified whole cell extracts (1% SDS) from the untransfected cells (Lane 1), non-specific scrambled siRNA transfected cells (Lane 2) and PPP1CC knockdown cells (Lane 3). The blot was probed with PP1 gamma Monoclonal Antibody (AF11F10) (Product # PA5-17250, 0.1 µg/mL) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to PPP1CC.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using PP1 gamma Monoclonal Antibody (AF11F10) (Product # MA5-17250) and a 36 kDa band corresponding to PPP1CC was observed across the cell lines and tissue tested. Whole cell Extracts of (30 µg lysate) of HeLa (Lane 1),NTERA-2 (Lane 2), HEK 293(Lane 3) and mouse lung (Lane 4 were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001).The blot was probed with the primary antibody (0.1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).