MA5-27471

antibody from Invitrogen Antibodies

Targeting: UQCRFS1 RIP1, RIS1, RISP, UQCR5

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27471

Provider

Invitrogen Antibodies

Product name

UQCRFS1 Monoclonal Antibody (OTI4H8)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

OTI4H8

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

High-throughput screening for natural compound-based autophagy modulators reveals novel chemotherapeutic mode of action for arzanol.

Deitersen J, Berning L, Stuhldreier F, Ceccacci S, Schlütermann D, Friedrich A, Wu W, Sun Y, Böhler P, Berleth N, Mendiburo MJ, Seggewiß S, Anand R, Reichert AS, Monti MC, Proksch P, Stork B
Cell death & disease 2021 May 31;12(6):560

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 6 Target identification for arzanol reveals OXPHOS complexes and possibly the F 1 F O ATP synthase/complex V as targets for arzanol. A Mass spectrometry (MS)-based target identification by affinity purification from HeLa cell whole lysates, and affinity purification and DARTS experiments from lysates of isolated mitochondria from HeLa cells reveal 125 novel potential targets of arzanol. B Cellular metabolism analysis was performed using a Seahorse Mito Stress Test Kit. Cells were incubated with arzanol for 15 min after measurement of the basal respiration and OCR was measured in at least octuplicates in three independent experiments. One representative experiment is shown (upper diagram). Respiration after treatment, maximal respiration, proton leak, and non-mitochondrial respiration were normalized to the basal respiration (lower diagram). Non-mitochondrial respiration = minimum rate measurement after rotenone/antimycin A injection; basal respiration = last rate measurement before first injection minus non-mitochondrial respiration; maximal respiration = maximum rate measurement after FCCP injection minus non-mitochondrial respiration; proton leak = minimum rate measurement after oligomycin injection minus non-mitochondrial respiration. C Arzanol inhibits mainly OXPHOS complexes II and III in a respiratory chain activity assay of isolated mitochondria after incubation for 15 min and 60 min. Complex activities were measured using the MitoCheck (r) kits in technical tripl