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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA800467 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800467, RRID:AB_2625470
- Product name
- WDR74 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2)
- Antibody type
- Monoclonal
- Description
- WDR74 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- WDR74
- Isotype
- IgG
- Antibody clone number
- OTI4B2
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references WDR74 participates in an early cleavage of the pre-rRNA processing pathway in cooperation with the nucleolar AAA-ATPase NVL2.
Hiraishi N, Ishida YI, Sudo H, Nagahama M
Biochemical and biophysical research communications 2018 Jan 1;495(1):116-123
Biochemical and biophysical research communications 2018 Jan 1;495(1):116-123
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-WDR74 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY WDR74 (RC221134, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-WDR74.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-WDR74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800467)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-WDR74 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA800467)
- Validation comment
- IHC
