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LearnHPA042816
antibody from Atlas Antibodies
Targeting: BRD2
BRD2-IT1, D6S113E, FSRG1, KIAA9001, NAT, RING3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- HPA042816 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA042816, RRID:AB_10794766
- Product name
- Anti-BRD2
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human BRD2, Gene description: bromodomain containing 2, Alternative Gene Names: D6S113E, FSRG1, KIAA9001, NAT, RING3, Validated applications: ICC, IHC, WB, Uniprot ID: P25440, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.05 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Interactome Rewiring Following Pharmacological Targeting of BET Bromodomains
The bromodomain inhibitor JQ1 triggers growth arrest and apoptosis in testicular germ cell tumoursin vitroandin vivo
Lambert J, Picaud S, Fujisawa T, Hou H, Savitsky P, Uusküla-Reimand L, Gupta G, Abdouni H, Lin Z, Tucholska M, Knight J, Gonzalez-Badillo B, St-Denis N, Newman J, Stucki M, Pelletier L, Bandeira N, Wilson M, Filippakopoulos P, Gingras A
Molecular Cell 2019;73(3):621-638.e17
Molecular Cell 2019;73(3):621-638.e17
The bromodomain inhibitor JQ1 triggers growth arrest and apoptosis in testicular germ cell tumoursin vitroandin vivo
Jostes S, Nettersheim D, Fellermeyer M, Schneider S, Hafezi F, Honecker F, Schumacher V, Geyer M, Kristiansen G, Schorle H
Journal of Cellular and Molecular Medicine 2016;21(7):1300-1314
Journal of Cellular and Molecular Medicine 2016;21(7):1300-1314
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Enhanced validation
- Submitted by
- klas2
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting BRD2 or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting BRD2 Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control
- Sample type
- U-2 OS
- Primary Ab dilution
- 1:138
- Conjugate
- Horseradish Peroxidase
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:3000
- Knockdown/Genetic Approaches Application
- Western blot
