A302-583A

antibody from Invitrogen Antibodies

Targeting: BRD2 BRD2-IT1, D6S113E, FSRG1, KIAA9001, NAT, RING3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A302-583A

Provider

Invitrogen Antibodies

Product name

BRD2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

0.20 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse BRD2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody (Product # A302-583A; lot A302-583A-6) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody (Product # A302-582A; lot A302-582A-6) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody A302-583A (lot A302-583A-5) used for IP at 6 µg per reaction. BRD2 was also immunoprecipitated by a previous lot of this antibody (lot A302-583A-4) and rabbit anti-BRD2 antibody A302-582A. For blotting immunoprecipitated BRD2, A302-583A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse BRD2 by western blot. Samples:Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD2 antibody A302-583A (lot A302-583A-5) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD2 by immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified rabbit anti- BRD2 (Cat. No.A302-583A Lot5) used at a dilution of 1:100 (2 µg/mL) (left) and rabbit anti-BRD2 recombinant monoclonal (A700-008-1) used at a dilution of 1:20 (right). Detection: Red-fluorescent goat anti-rabbit IgG cross-adsorbed Antibody DyLight 594 conjugated.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BRD2 (Cat. No.A302-583A Lot5) used at a dilution of 1:200 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD2 antibody (Product # A302-583A; lot A302-583A-6) used for IP at 6 µg per reaction. BRD2 was also immunoprecipitated by a previous lot of this antibody (lot A302-583A-5) and rabbit anti-BRD2 antibody (Product # A302-582A). For blotting immunoprecipitated BRD2, Product # A302-583A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.