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LearnA700-008
antibody from Invitrogen Antibodies
Targeting: BRD2
BRD2-IT1, D6S113E, FSRG1, KIAA9001, NAT, RING3
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [1]
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- Product number
- A700-008 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRD2 Recombinant Rabbit Monoclonal Antibody (BL-167-2A2)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BL-167-2A2
- Vial size
- 100 µL
- Concentration
- 40 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse BRD2 by western blot. Samples: Whole cell lysate (50 µg) from RenCa, NIH 3T3, and C2C12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (Product # A700-008 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (Product # A700-008 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin (Product # A300-491A).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD2 by WB of immunoprecipitates from 293T lysate. Antibodies: Rabbit anti-BRD2 recombinant monoclonal [BL-167-2A2] (A700-008) and rabbit anti-BRD2 antibodies (A302-582A, A302-583A). Secondary: ReliaBLOT® reagents (WB120).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD2 by WB in 293T, HeLa, SW620, SK-MEL-28 (SK), Jurkat, MCF7, HepG2 and A549 lysate. Antibody: Rabbit anti-BRD2 recombinant monoclonal [BL-167-2A2] (A700-008). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD2 recombinant monoclonal antibody [BL-167-2A2] (Product # A700-008; lot 2) used for IP at 20 µL/mg lysate. BRD2 was also immunoprecipitated by a previous lot of this antibody (lot A700-008-1) and rabbit anti-BRD2 antibody (Product # A302-583A). For blotting immunoprecipitated BRD2, Product # A700-008 was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.
