- PA5-36472 - Invitrogen Antibodies CBLN4 antibody

Submitted references Transcriptional profiling aligned with in situ expression image analysis reveals mosaically expressed molecular markers for GABA neuron sub-groups in the ventral tegmental area.

Paul EJ, Tossell K, Ungless MA
The European journal of neuroscience 2019 Dec;50(11):3732-3749

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Cerebellin 4 using Cerebellin 4 polyclonal antibody (Product # PA5-36472) at a dilution of 1:500. Lane 1: HEK293T cell lysate, Lane 2: sp2/0 cell lysate, Lane 3: Rat liver tissue lysate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of Cerebellin 4 in Lane 1: the Brain tissue lysate of mouse (40 µg), Lane 2: the Brain tissue lysate of rat (40 µg), Lane 3: U-87MG whole cell lysate (20 µg), Lane 4: L02 whole cell lysate (20 µg). Samples were incubated with Cerebellin 4 polyclonal antibody (Product # PA5-36472) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Isolation and sequencing of RNA from samples enriched for either GABA or dopamine neurons. (a) Representative images showing triple immunolabelling for HA , GABAAR alpha1 and TH in the VTA from a VGATC re:RiboTag mouse (top panels) and double immunofluorescence for HA and TH in the VTA from a DATC re:RiboTag mouse (bottom panels). In each case HA immunoreactivity is selectively localised to the target neuronal population as illustrated by co-localisation with GABAAR alpha1 (in HA +/ TH - cells (yellow arrows) but not in TH + cells (blue arrows)) in the top panels and TH in the bottom panels. (b) Graph showing mean (+ SEM ) percentage of cells ( n = 481) that were either HA +, TH +, or HA + and TH +. (c) Graph showing mean (+ SEM ) percentage of HA + ( TH -) cells ( n = 278) that expressed GABAAR alpha1. (d) Graph showing mean (+ SEM ) GABA neuron-specific transcripts (red box; slc32a1, Gad1, Gad2, Gabra1, Pvalb ) significantly enriched in samples enriched for GABA neurons compared to dopamine neurons ( P-Adj < 0.05). In contrast, dopamine neuron-related transcripts ( green box; Dat, Th, Aadc) were not enriched ( P-Adj > 0.05). (e) Heatmap depicting the 15,015 mRNA transcripts detected in GABA and dopamine neurons (each row represents a different transcript). Expression levels (reads per kilobase of transcript per million mapped reads; RPKM ) were scaled and sorted by z-score. The red-green scale illustrates level of enrichment (red = enriched; green = de-enriched). E

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4 CBLN 4 expression in GABA neurons in the VTA . a, Left panel shows ISH image from the Allen Mouse Brain Atlas showing mosaic expression of CBLN 4 in the PN and medial PBP . b, Graph showing mean (+ SEM ) percentage of CBLN + cells ( n = 59 cells) that express either HA , or TH , or HA and TH , or neither. c, Graph showing mean (+ SEM ) percentage of HA + cells ( n = 124 cells) that express CBLN and the percentage of TH + cells ( n = 94 cells) that express CBLN . d, e, Representative examples of immunolabelling of CBLN 4 (magenta), HA (blue) and TH (green) in the VTA of a VGATC re:RiboTag mouse, illustrating exemplar cells exhibiting CBLN 4 co-localisation with HA , but not TH (yellow arrows). f, Representative example of immunolabelling in the SN c. [Colour figure can be viewed at http://www.wileyonlinelibrary.com ]

PA5-36472

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