Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
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- Product number
- PA5-18321 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EFHD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 128,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Lymphnode lysate using Product # PA5-18321 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Swiprosin 1/EFHD2 in Human Tonsil (A), Human Thymus (B) and Human Lymph Node (C) and of Mouse Thymus (D) and Mouse Lymph Node (E) lysates (35µg protein in RIPA buffer). Samples were probed with the Swiprosin 1/EFHD2 antibody (Product # PA5-18321, 0.3µg/mL) for 1 hour. Western blot was detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of EFHD2 by a EFHD2 monoclonal antibody (Product # PA5-18321) at a concentration of 0.5 µg/mL. Human Thymus, Human Lymph node and Human Tonsil lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of Human Lymphnode lysate using Product # PA5-18321 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin embedded human tonsil using Product # PA5-18321 at a concentration of 0.3 µg/mL. The tissue was processed by microwaved antigen retrieval with citrate buffer pH 6 and stained with HRP.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Swiprosin 1/EFHD2 in paraffin embedded Human Tonsil tissue. Steamed antigen retrieval was performed with citrate buffer pH 6, followed by application of the Swiprosin 1/EFHD2 antibody (Product # PA5-18321) (2µg/mL) and HRP-staining.