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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA045038 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA045038, RRID:AB_10959457
- Product name
- Anti-HLA-DOA
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human HLA-DOA, Gene description: major histocompatibility complex, class II, DO alpha, Alternative Gene Names: HLA-D0-alpha, HLA-DNA, HLA-DZA, Validated applications: WB, IHC, Uniprot ID: P06340, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.4 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Two histologically colorectal carcinomas subsets from the serrated pathway show different methylome signatures and diagnostic biomarkers
García-Solano J, Turpin M, Torres-Moreno D, Huertas-López F, Tuomisto A, Mäkinen M, Conesa A, Conesa-Zamora P
Clinical Epigenetics 2018;10(1)
Clinical Epigenetics 2018;10(1)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and HLA-DOA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419523).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human tonsil and liver tissues using HPA045038 antibody. Corresponding HLA-DOA RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
