PA5-59903

antibody from Invitrogen Antibodies

Targeting: CIAPIN1 Anamorsin, CIAE2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-59903

Provider

Invitrogen Antibodies

Product name

CIAPIN1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KLWTLSANDM EDDSMDLIDS DELLDPEDLK KPDPASLRAA SCGEGKKRKA CKNCTCGLAE ELEKEKSREQ MSSQPKSACG NCYLGDAFRC ASCPYLGMPA FKPGEKVLLS D Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 92%.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Downregulation of miR‑16 protects H9c2(2‑1) cells against hypoxia/reoxygenation damage by targeting CIAPIN1 and regulating the NF‑κB pathway.

Zhang HJ, Zhang YN, Teng ZY
Molecular medicine reports 2019 Oct;20(4):3113-3122

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Anamorsin in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp. Samples were probed using an Anamorsin Polyclonal Antibody (Product # PA5-59903).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Anamorsin in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an Anamorsin Polyclonal Antibody (Product # PA5-59903).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of Anamorsin in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using an Anamorsin Polyclonal Antibody (Product # PA5-59903).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7. Effect of CIAPIN1 overexpression on the expression of apoptosis-associated proteins in H9c2 cells following H/R. (A) mRNA expression levels of CIAPIN1 in H9c2 cells transfected with pcDNA3.1-CIAPIN1 compared with cells transfected with the empty vector. **P