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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-29259 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CIAPIN1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: Jurkat, Raji, K562, THP-1, HL-60, NCI-H929, NIH-3T3, JC, BCL-1. Predicted reactivity: Mouse (81%), Rat (81%), Chimpanzee (99%), Bovine (90%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references New prognostic markers revealed by RNA-Seq transcriptome analysis after MYC silencing in a metastatic gastric cancer cell line.
Lopes LO, Maués JH, Ferreira-Fernandes H, Yoshioka FK, Júnior SCS, Santos AR, Ribeiro HF, Rey JA, Soares PC, Burbano RR, Pinto GR
Oncotarget 2019 Oct 8;10(56):5768-5779
Oncotarget 2019 Oct 8;10(56):5768-5779
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CIAPIN1 Polyclonal Antibody (Product # PA5-29259). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 10% SDS PAGE. CIAPIN1 Polyclonal Antibody (Product # PA5-29259) diluted at 1:3,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CIAPIN1 Polyclonal Antibody (Product # PA5-29259). Sample (30 µg of whole cell lysate). Lane A: Jurkat . Lane B: Raji. Lane C: K562. Lane D: THP-1. Lane E: HL-60. Lane F: NCI-H929. 10% SDS PAGE. CIAPIN1 Polyclonal Antibody (Product # PA5-29259) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CIAPIN1 Polyclonal Antibody detects CIAPIN1 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CIAPIN1 protein stained by CIAPIN1 Polyclonal Antibody (Product # PA5-29259) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CIAPIN1 was performed in paraffin-embedded rat adrenal gland tissue using CIAPIN1 Polyclonal Antibody (Product # PA5-29259) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CIAPIN1 Polyclonal Antibody detects CIAPIN1 protein at cytosol and nucleus on SkHep1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1 xenograft. CIAPIN1 Polyclonal Antibody (Product # PA5-29259) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
