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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- PA5-85574 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TLX3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (100%), Xenopus laevis (100%), Chicken (100%), Bovine (100%). Positive Control: SK-N-SH, IMR32, SK-N-AS. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TLX3 in A) SK-N-SH whole cell lysate, B) IMR32 whole cell lysate, C) SK-N-AS whole cell lysate using TLX3 polyclonal antibody (Product # PA5-85574) using 30 µg of sample at a dilution of 1:1000. Sample was then incubated with Hoechst secondary antibody. Prior to incubation with primary antibody, the sample was separated on 12% SDS-PAGE.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TLX3 in paraffin-embedded mouse muscle using TLX3 polyclonal antibody (Product # PA5-85574) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TLX3 Polyclonal Antibody detects TLX3 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. TLX3 Polyclonal Antibody (Product # PA5-85574) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TLX3 in paraffin-embedded mouse fore brain using TLX3 polyclonal antibody (Product # PA5-85574) at a dilution of 1:500.
