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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29417 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-SPDYA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- PA5-29417 targets SPDYA in IHC (P) and WB applications and shows reactivity with Human samples. The PA5-29417 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 313 of Human SPDYA. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references The cyclin-like protein, SPY1, regulates the ERĪ± and ERK1/2 pathways promoting tamoxifen resistance.
Ferraiuolo RM, Tubman J, Sinha I, Hamm C, Porter LA
Oncotarget 2017 Apr 4;8(14):23337-23352
Oncotarget 2017 Apr 4;8(14):23337-23352
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPDYA using 30 µg of A) NT2D1 (B) PC-3 (C) U87-MG and D) SK-N-SH lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SPDYA polyclonal antibody (Product # PA5-29417) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SPDYA in paraffin-embedded BT483 xenograft using a SPDYA polyclonal antibody (Product # PA5-29417) at a dilution of 1:1000.
