PA5-83607

antibody from Invitrogen Antibodies

Targeting: NCBP1 CBP80, NCBP, Sto1

Western blot (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-83607

Provider

Invitrogen Antibodies

Product name

NCBP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: FVSVTQEEDV PQVRRDWYVY AFLSSLPWVG KELYEKKDAE MDRIFANTES YLKRRQKTHV PMLQVWTADK PHPQEEYLDC LWAQIQKLKK DRWQERHILR PYLAFDSILC EALQHNLPPF TPPPH

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

2'-O-Methylation of the second transcribed nucleotide within the mRNA 5' cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion.

Drazkowska K, Tomecki R, Warminski M, Baran N, Cysewski D, Depaix A, Kasprzyk R, Kowalska J, Jemielity J, Sikorski PJ
Nucleic acids research 2022 Sep 9;50(16):9051-9071

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NCBP1 in A-431 cells using a NCBP1 polyclonal antibody (Product # PA5-83607). The analysis shows localization to nucleoplasm & cytosol.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNalpha pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value +- SEM normalized to protein production in mock-treated cells. Statistical significance: * P