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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [23]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- V3374 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HLA-DP + HLA-DQ + HLA-DR Antibody (MHC II)
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- CR3/43
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the HLA-DP/DQ/DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Inhomogeneous distribution of Iba-1 characterizes microglial pathology in Alzheimer's disease.
Studying Human Brain Inflammation in Leptomeningeal and Choroid Plexus Explant Cultures.
Expression of microRNAs miR21, miR146a, and miR155 in tuberous sclerosis complex cortical tubers and their regulation in human astrocytes and SEGA-derived cell cultures.
HLA-G and MHC Class II Protein Expression in Diffuse Large B-Cell Lymphoma.
Proposed Diagnostic Criteria for Chronic Antibody-Mediated Rejection in Liver Allografts.
Dysregulation of the (immuno)proteasome pathway in malformations of cortical development.
Down-regulation of IKKβ expression in glioma-infiltrating microglia/macrophages is associated with defective inflammatory/immune gene responses in glioblastoma.
Complement C1q-C3-associated synaptic changes in multiple sclerosis hippocampus.
Saccadic Palsy following Cardiac Surgery: Possible Role of Perineuronal Nets.
Human leukocyte antigen DR surface expression on CD14+ monocytes during adverse events after hematopoietic stem cell transplantation.
Deep-brain stimulation associates with improved microvascular integrity in the subthalamic nucleus in Parkinson's disease.
Expression of neurodegenerative disease-related proteins and caspase-3 in glioneuronal tumours.
Distinct binding of amyloid imaging ligands to unique amyloid-β deposited in the presubiculum of Alzheimer's disease.
Metabotropic glutamate receptor 5 in Down's syndrome hippocampus during development: increased expression in astrocytes.
Endothelin-1 is over-expressed in amyotrophic lateral sclerosis and induces motor neuron cell death.
Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
Progressive neurodegeneration after experimental brain trauma: association with chronic microglial activation.
BRAF V600E mutation is associated with mTOR signaling activation in glioneuronal tumors.
Cell injury and premature neurodegeneration in focal malformations of cortical development.
Severe Neonatal Epileptic Encephalopathy and KCNQ2 Mutation: Neuropathological Substrate?
Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures.
Spinal cord injury causes brain inflammation associated with cognitive and affective changes: role of cell cycle pathways.
Correlation between clinical and histologic findings in the human neonatal hippocampus after perinatal asphyxia.
Tischer J, Krueger M, Mueller W, Staszewski O, Prinz M, Streit WJ, Bechmann I
Glia 2016 Sep;64(9):1562-72
Glia 2016 Sep;64(9):1562-72
Studying Human Brain Inflammation in Leptomeningeal and Choroid Plexus Explant Cultures.
Dragunow M, Feng S, Rustenhoven J, Curtis M, Faull R
Neurochemical research 2016 Mar;41(3):579-88
Neurochemical research 2016 Mar;41(3):579-88
Expression of microRNAs miR21, miR146a, and miR155 in tuberous sclerosis complex cortical tubers and their regulation in human astrocytes and SEGA-derived cell cultures.
van Scheppingen J, Iyer AM, Prabowo AS, Mühlebner A, Anink JJ, Scholl T, Feucht M, Jansen FE, Spliet WG, Krsek P, Zamecnik J, Buccoliero AM, Giordano F, Genitori L, Kotulska K, Jozwiak S, Jaworski J, Liszewska E, van Vliet EA, Aronica E
Glia 2016 Jun;64(6):1066-82
Glia 2016 Jun;64(6):1066-82
HLA-G and MHC Class II Protein Expression in Diffuse Large B-Cell Lymphoma.
Jesionek-Kupnicka D, Bojo M, Prochorec-Sobieszek M, Szumera-Ciećkiewicz A, Jabłońska J, Kalinka-Warzocha E, Kordek R, Młynarski W, Robak T, Warzocha K, Lech-Maranda E
Archivum immunologiae et therapiae experimentalis 2016 Jun;64(3):225-40
Archivum immunologiae et therapiae experimentalis 2016 Jun;64(3):225-40
Proposed Diagnostic Criteria for Chronic Antibody-Mediated Rejection in Liver Allografts.
O'Leary JG, Cai J, Freeman R, Banuelos N, Hart B, Johnson M, Jennings LW, Kaneku H, Terasaki PI, Klintmalm GB, Demetris AJ
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2016 Feb;16(2):603-14
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2016 Feb;16(2):603-14
Dysregulation of the (immuno)proteasome pathway in malformations of cortical development.
van Scheppingen J, Broekaart DW, Scholl T, Zuidberg MR, Anink JJ, Spliet WG, van Rijen PC, Czech T, Hainfellner JA, Feucht M, Mühlebner A, van Vliet EA, Aronica E
Journal of neuroinflammation 2016 Aug 26;13(1):202
Journal of neuroinflammation 2016 Aug 26;13(1):202
Down-regulation of IKKβ expression in glioma-infiltrating microglia/macrophages is associated with defective inflammatory/immune gene responses in glioblastoma.
Mieczkowski J, Kocyk M, Nauman P, Gabrusiewicz K, Sielska M, Przanowski P, Maleszewska M, Rajan WD, Pszczolkowska D, Tykocki T, Grajkowska W, Kotulska K, Roszkowski M, Kostkiewicz B, Kaminska B
Oncotarget 2015 Oct 20;6(32):33077-90
Oncotarget 2015 Oct 20;6(32):33077-90
Complement C1q-C3-associated synaptic changes in multiple sclerosis hippocampus.
Michailidou I, Willems JG, Kooi EJ, van Eden C, Gold SM, Geurts JJ, Baas F, Huitinga I, Ramaglia V
Annals of neurology 2015 Jun;77(6):1007-26
Annals of neurology 2015 Jun;77(6):1007-26
Saccadic Palsy following Cardiac Surgery: Possible Role of Perineuronal Nets.
Eggers SD, Horn AK, Roeber S, Härtig W, Nair G, Reich DS, Leigh RJ
PloS one 2015 Jul 2;10(7):e0132075
PloS one 2015 Jul 2;10(7):e0132075
Human leukocyte antigen DR surface expression on CD14+ monocytes during adverse events after hematopoietic stem cell transplantation.
Döring M, Rohrer KM, Erbacher A, Gieseke F, Schwarze CP, Bader P, Handgretinger R, Hofbeck M, Kerst G
Annals of hematology 2015 Feb;94(2):265-73
Annals of hematology 2015 Feb;94(2):265-73
Deep-brain stimulation associates with improved microvascular integrity in the subthalamic nucleus in Parkinson's disease.
Pienaar IS, Lee CH, Elson JL, McGuinness L, Gentleman SM, Kalaria RN, Dexter DT
Neurobiology of disease 2015 Feb;74:392-405
Neurobiology of disease 2015 Feb;74:392-405
Expression of neurodegenerative disease-related proteins and caspase-3 in glioneuronal tumours.
Prabowo AS, Iyer AM, Veersema TJ, Anink JJ, Schouten-van Meeteren AY, Spliet WG, van Rijen PC, Ferrier CH, Thom M, Aronica E
Neuropathology and applied neurobiology 2015 Feb;41(2):e1-e15
Neuropathology and applied neurobiology 2015 Feb;41(2):e1-e15
Distinct binding of amyloid imaging ligands to unique amyloid-β deposited in the presubiculum of Alzheimer's disease.
Ji B, Chen CJ, Bando K, Ashino H, Shiraishi H, Sano H, Kasahara H, Minamizawa T, Yamada K, Ono M, Zhang MR, Seki C, Farde L, Suhara T, Higuchi M
Journal of neurochemistry 2015 Dec;135(5):859-66
Journal of neurochemistry 2015 Dec;135(5):859-66
Metabotropic glutamate receptor 5 in Down's syndrome hippocampus during development: increased expression in astrocytes.
Iyer AM, van Scheppingen J, Milenkovic I, Anink JJ, Lim D, Genazzani AA, Adle-Biassette H, Kovacs GG, Aronica E
Current Alzheimer research 2014;11(7):694-705
Current Alzheimer research 2014;11(7):694-705
Endothelin-1 is over-expressed in amyotrophic lateral sclerosis and induces motor neuron cell death.
Ranno E, D'Antoni S, Spatuzza M, Berretta A, Laureanti F, Bonaccorso CM, Pellitteri R, Longone P, Spalloni A, Iyer AM, Aronica E, Catania MV
Neurobiology of disease 2014 May;65:160-71
Neurobiology of disease 2014 May;65:160-71
Evidence for mTOR pathway activation in a spectrum of epilepsy-associated pathologies.
Liu J, Reeves C, Michalak Z, Coppola A, Diehl B, Sisodiya SM, Thom M
Acta neuropathologica communications 2014 Jul 8;2:71
Acta neuropathologica communications 2014 Jul 8;2:71
Progressive neurodegeneration after experimental brain trauma: association with chronic microglial activation.
Loane DJ, Kumar A, Stoica BA, Cabatbat R, Faden AI
Journal of neuropathology and experimental neurology 2014 Jan;73(1):14-29
Journal of neuropathology and experimental neurology 2014 Jan;73(1):14-29
BRAF V600E mutation is associated with mTOR signaling activation in glioneuronal tumors.
Prabowo AS, Iyer AM, Veersema TJ, Anink JJ, Schouten-van Meeteren AY, Spliet WG, van Rijen PC, Ferrier CH, Capper D, Thom M, Aronica E
Brain pathology (Zurich, Switzerland) 2014 Jan;24(1):52-66
Brain pathology (Zurich, Switzerland) 2014 Jan;24(1):52-66
Cell injury and premature neurodegeneration in focal malformations of cortical development.
Iyer A, Prabowo A, Anink J, Spliet WG, van Rijen PC, Aronica E
Brain pathology (Zurich, Switzerland) 2014 Jan;24(1):1-17
Brain pathology (Zurich, Switzerland) 2014 Jan;24(1):1-17
Severe Neonatal Epileptic Encephalopathy and KCNQ2 Mutation: Neuropathological Substrate?
Dalen Meurs-van der Schoor C, van Weissenbruch M, van Kempen M, Bugiani M, Aronica E, Ronner H, Vermeulen RJ
Frontiers in pediatrics 2014 Dec 19;2:136
Frontiers in pediatrics 2014 Dec 19;2:136
Recovery of extracellular vesicles from human breast milk is influenced by sample collection and vesicle isolation procedures.
Zonneveld MI, Brisson AR, van Herwijnen MJ, Tan S, van de Lest CH, Redegeld FA, Garssen J, Wauben MH, Nolte-'t Hoen EN
Journal of extracellular vesicles 2014 Aug 14;3
Journal of extracellular vesicles 2014 Aug 14;3
Spinal cord injury causes brain inflammation associated with cognitive and affective changes: role of cell cycle pathways.
Wu J, Zhao Z, Sabirzhanov B, Stoica BA, Kumar A, Luo T, Skovira J, Faden AI
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Aug 13;34(33):10989-1006
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Aug 13;34(33):10989-1006
Correlation between clinical and histologic findings in the human neonatal hippocampus after perinatal asphyxia.
Schiering IA, de Haan TR, Niermeijer JM, Koelman JH, Majoie CB, Reneman L, Aronica E
Journal of neuropathology and experimental neurology 2014 Apr;73(4):324-34
Journal of neuropathology and experimental neurology 2014 Apr;73(4):324-34
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Raji cell lysate with HLA-DP/DQ/DR antibody (clone CR3/43). Expected molecular weight ~36 kDa (alpha chain) and ~27 kDa (beta chain).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free HLA-DP/DQ/DR Antibody (clone CR3/43). Confirmation of Integrity and Purity of the Antibody.
