328232

antibody from BioLegend

Targeting: ENTPD1 ATPDase, CD39, NTPDase-1, SPG64

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

328232

Provider

BioLegend

Proper citation

BioLegend Cat#328232, RRID:AB_2686985

Product name

Alexa Fluor® 594 anti-human CD39

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

A1

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunohistochemistry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human paraffin-embedded placenta tissue slice was prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of anti-human CD39 (clone A1) Alexa Fluor® 594 (red) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.

Conjugate

Yellow dye