Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB3408 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3408, RRID:AB_1574443
- Product name
- DOK4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of DOK4.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Dok-4 is a novel negative regulator of T cell activation.
Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5.
DOK4 and DOK5: new Dok-related genes expressed in human T cells.
Gérard A, Ghiotto M, Fos C, Guittard G, Compagno D, Galy A, Lemay S, Olive D, Nunès JA
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7681-9
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 15;182(12):7681-9
Two new substrates in insulin signaling, IRS5/DOK4 and IRS6/DOK5.
Cai D, Dhe-Paganon S, Melendez PA, Lee J, Shoelson SE
The Journal of biological chemistry 2003 Jul 11;278(28):25323-30
The Journal of biological chemistry 2003 Jul 11;278(28):25323-30
DOK4 and DOK5: new Dok-related genes expressed in human T cells.
Favre C, Gérard A, Clauzier E, Pontarotti P, Olive D, Nunès JA
Genes and immunity 2003 Jan;4(1):40-5
Genes and immunity 2003 Jan;4(1):40-5
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of DOK4 (arrow) using DOK4 polyclonal antibody (Cat # PAB3408).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DOK4 gene (Lane 2).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DOK4 polyclonal antibody (Cat # PAB3408) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
