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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
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- Product number
- PA5-87381 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DOK4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MATNFSDIVK QGYVKMKSRK LGIYRRCWLV FRKSSSKGPQ RLEKYPDEKS VCLRGCPKVT EISNVKCVTR LPKETKRQAV AIIFTDDSAR TFTCDSELEA EEWYKTLSVE CLGSRLNDIS LGEPDLLAPG VQCEQTDRFN VFLLPCPNLD VYGECKLQIT HENIYLWDIH NPRVKLVSWP LCSLRRYGRD ATRFTFEAGR MCDAGEGLYT FQTQEGEQIY QRVHSATLAI AEQHKRVLLE MEKNVRLLNK GTEHYSYPCT PTTMLPRSAY WHHITGSQNI AEASSYAGEG YGAAQASSET DLLNRFILLK PKPSQGDSSE AKTPSQ Positive Samples: MCF7
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.09 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DOK4 in extracts of various cell lines. Samples were incubated with DOK4 Polyclonal antibody (Product # PA5-87381) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DOK4 was performed in HeLa cells using DOK4 Polyclonal Antibody (Product # PA5-87381).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DOK4 in NIH/3T3 cells. Samples were incubated with DOK4 Polyclonal antibody (Product # PA5-87381) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DOK4 in PC-12 cells. Samples were incubated with DOK4 Polyclonal antibody (Product # PA5-87381) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DOK4 in SH-SY5Y cells. Samples were incubated with DOK4 Polyclonal antibody (Product # PA5-87381) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DOK4 in paraffin-embedded human colon carcinoma. Samples were incubated with DOK4 Polyclonal antibody (Product # PA5-87381) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
