MA5-27738

antibody from Invitrogen Antibodies

Targeting: COL11A2 DFNA13, DFNB53, HKE5

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27738

Provider

Invitrogen Antibodies

Product name

COL11A2 Monoclonal Antibody (GT473)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1) Predicted Reactivity: Mouse (85%), Rat (85%), Bovine (93%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

GT473

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Transcriptional profiling of intervertebral disc in a post-traumatic early degeneration organ culture model.

Cui S, Zhou Z, Chen X, Wei F, Richards RG, Alini M, Grad S, Li Z
JOR spine 2021 Sep;4(3):e1146

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COL11A2 Monoclonal Antibody (GT473) detects COL11A2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COL11A2 protein stained by COL11A2 Monoclonal Antibody (GT473) (Product # MA5-27738) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COL11A2 Monoclonal Antibody (GT473) detects COL11A2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: COL11A2 protein stained by COL11A2 Monoclonal Antibody (GT473) (Product # MA5-27738) diluted at 1:500. Blue: Hoechst 33342 staining.