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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
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- Product number
- MA5-27738 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL11A2 Monoclonal Antibody (GT473)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1) Predicted Reactivity: Mouse (85%), Rat (85%), Bovine (93%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT473
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Transcriptional profiling of intervertebral disc in a post-traumatic early degeneration organ culture model.
Cui S, Zhou Z, Chen X, Wei F, Richards RG, Alini M, Grad S, Li Z
JOR spine 2021 Sep;4(3):e1146
JOR spine 2021 Sep;4(3):e1146
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COL11A2 Monoclonal Antibody (GT473) detects COL11A2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1).12 % SDS-PAGE. COL11A2 Monoclonal Antibody (GT473) (Product # MA5-27738) dilution: 1:5,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of COL11A2 was performed by separating 50 µg of various tissue extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a COL11A2 Monoclonal Antibody (GT473) (Product # MA5-27738) at a dilution of 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COL11A2 Monoclonal Antibody (GT473) detects COL11A2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1).12 % SDS-PAGE. COL11A2 Monoclonal Antibody (GT473) (Product # MA5-27738) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COL11A2 Monoclonal Antibody (GT473) detects COL11A2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: COL11A2 protein stained by COL11A2 Monoclonal Antibody (GT473) (Product # MA5-27738) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COL11A2 Monoclonal Antibody (GT473) detects COL11A2 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: COL11A2 protein stained by COL11A2 Monoclonal Antibody (GT473) (Product # MA5-27738) diluted at 1:500. Blue: Hoechst 33342 staining.
