TA502347

antibody from OriGene

Targeting: RTF2 C20orf43, CDAO5, HSPC164, RTFDC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502347

Provider

OriGene

Proper citation

OriGene Cat#TA502347, RRID:AB_11125212

Product name

C20orf43 mouse monoclonal antibody, clone OTI3A5 (formerly 3A5)

Antibody type

Monoclonal

Description

C20orf43 mouse monoclonal antibody, clone OTI3A5 (formerly 3A5)

Host

Mouse

Conjugate

Unconjugated

Epitope

RTF2

Isotype

IgG

Antibody clone number

OTI3A5

Vial size

100 µl

Concentration

0.24 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C20orf43 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C20orf43 (RC201652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C20orf43.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-C20orf43 mouse monoclonal antibody (TA502347) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C20orf43(RC201652).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC201652 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-C20orf43 antibody(TA502347), and then analyzed by flow cytometry.

Validation comment

FC