A02831-5
antibody from Boster Biological Technology
Targeting: FOXE1
FKHL15, FOXE2, HFKH4, TITF2, TTF-2
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A02831-5 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-FOXE1 Picoband™ Antibody
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for FOXE1 detection. Tested with WB, FCM, Direct ELISA in Human.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Disruption of the foxe1 gene in zebrafish reveals conserved functions in development of the craniofacial skeleton and the thyroid.
Raterman ST, Von Den Hoff JW, Dijkstra S, De Vriend C, Te Morsche T, Broekman S, Zethof J, De Vrieze E, Wagener FADTG, Metz JR
Frontiers in cell and developmental biology 2023;11:1143844
Frontiers in cell and developmental biology 2023;11:1143844
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of FOXE1 using anti-FOXE1 antibody (A02831-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXE1 antigen affinity purified polyclonal antibody (Catalog # A02831-5) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FOXE1 at approximately 41KD. The expected band size for FOXE1 is at 38KD.
- Additional image