Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000306-M12 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000306-M12, RRID:AB_605929
- Product name
- ANXA3 monoclonal antibody (M12), clone 4F1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ANXA3.
- Antigen sequence
MASIWVGHRGTVRDYPDFSPSVDAEAIQKAIRGIG
TDEKMLISILTERSNAQRQLIVKEYQAAYGKELKD
DLKGDLSGHFEHLMVALVTPPAVFDAKQLKKSMKG
AGTNEDALIEILTTRTSRQMKDISQAYYTVYKKSL
GDDISSETSGDFRKALLTLADGRRDESLKVDEHLA
KQDAQILYKAGENRWGTDEDKFTEILCLRSFPQLK
LTFDEYRNISQKDIVDSIKGELSGHFEDLLLAIVN
CVRNTPAFLAERLHRALKGIGTDEFTLNRIMVSRS
EIDLLDIRTEFKKHYGYSLYSAIKSDTSGDYEITL
LKICGGDD- Isotype
- IgG
- Antibody clone number
- 4F1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Annexin A3 as a negative regulator of adipocyte differentiation.
A multiplex model of combining gene-based, protein-based, and metabolite-based with positive and negative markers in urine for the early diagnosis of prostate cancer.
Watanabe T, Ito Y, Sato A, Hosono T, Niimi S, Ariga T, Seki T
Journal of biochemistry 2012 Oct;152(4):355-63
Journal of biochemistry 2012 Oct;152(4):355-63
A multiplex model of combining gene-based, protein-based, and metabolite-based with positive and negative markers in urine for the early diagnosis of prostate cancer.
Cao DL, Ye DW, Zhang HL, Zhu Y, Wang YX, Yao XD
The Prostate 2011 May 15;71(7):700-10
The Prostate 2011 May 15;71(7):700-10
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody (M12), clone 4F1.Lane 1: ANXA3 transfected lysate(37.145 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ANXA3 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol