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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Western blot [2]
- ELISA [1]
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- Product number
- H00000306-M12 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000306-M12, RRID:AB_828585
- Product name
- Mouse Monoclonal Annexin A3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ANXA3 (4F1)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Annexin A3 as a negative regulator of adipocyte differentiation.
A multiplex model of combining gene-based, protein-based, and metabolite-based with positive and negative markers in urine for the early diagnosis of prostate cancer.
Watanabe T, Ito Y, Sato A, Hosono T, Niimi S, Ariga T, Seki T
Journal of biochemistry 2012 Oct;152(4):355-63
Journal of biochemistry 2012 Oct;152(4):355-63
A multiplex model of combining gene-based, protein-based, and metabolite-based with positive and negative markers in urine for the early diagnosis of prostate cancer.
Cao DL, Ye DW, Zhang HL, Zhu Y, Wang YX, Yao XD
The Prostate 2011 May 15;71(7):700-10
The Prostate 2011 May 15;71(7):700-10
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12] - Western blot analysis of ANXA3 over-expressed 293 cell line. Lane 2 cotransfected with ANXA3 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000306-M12. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Annexin A3 Antibody (4F1) [H00000306-M12] - Analysis of ANXA3 expression in transfected 293T cell line by ANXA3 monoclonal antibody (M12), clone 4F1. Lane 1: ANXA3 transfected lysatE (37.145 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Annexin A3 Antibody (4F1) [H00000306-M12] - Detection limit for recombinant GST tagged ANXA3 is approximately 1ng/ml as a capture antibody.
