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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- F54290 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- NGN3 Antibody / Neurogenin 3
- Antibody type
- Polyclonal
- Description
- This highly specific NGN3 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence applications with human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 ml, 0.4 ml
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the NGN3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human ThP-1 cell lysate with NGN3 antibody. Predicted molecular weight ~23 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) DU145, 2) LNCaP, 3) PANC1 and 4) PC-3 cell lysate with NGN3 antibody. Predicted molecular weight ~23 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human NCI-H460 cells with NGN3 antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human small intestine tissue with NGN3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human pancreas tissue with NGN3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
