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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- CF500346 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEUROG3 Monoclonal Antibody (OTI3B5), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI3B5
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (RC224767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUR. Positive lysates LY402827 (100 µg) and LC402827 (20 µg) can be purchased separately from OriGene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (RC224767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUR. Positive lysates LY402827 (100 µg) and LC402827 (20 µg) can be purchased separately from OriGene.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Anti-NEUROG3 mouse monoclonal antibody (NEUROG3) Immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY NEUROG3 (RC224767).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human lymph node tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (TA500346) at 1:150 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of NEUROG3 by using TrueMab monoclonal anti-NEUROG3 antibody (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 µg of anti-NEUROG3 antibody and 20ul (0.1mg) of goat anti-mouse conj µgated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
