- MA5-34789 - Invitrogen Antibodies IQGAP1 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of IQGAP1 in Lane 1: HUVEC, Lane 2: A431, Lane 3: Mouse placenta . Samples were incubated with IQGAP1 monoclonal antibody (Product # MA5-34789), at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of IQGAP1 in Lane 1: HUVEC, Lane 2: A431, Lane 3: Mouse placenta . Samples were incubated with IQGAP1 monoclonal antibody (Product # MA5-34789), at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-IQGAP1 Recombinant Rabbit Monoclonal Antibody (JG38-74) (Product # MA5-34789) and a 189 kDa band corresponding to IQGAP1 was observed across all cell lysates tested. Whole cell extracts (60 µg lysate) of HaCaT (Lane 1), THP-1 (Lane 2), K-562 (Lane 3), HEK-293 (Lane 4), A-431 (Lane 5), NIH/3T3 (Lane 6) and Hep G2 (Lane 7) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). An uncharacterized band (*) was observed at ~70 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of IQGAP1 was achieved by transfecting HEK-293 with IQGAP1 specific siRNAs (Silencer® select Product # s16837, s16839). Western blot analysis (Fig. a) was performed using Whole cell extracts from the IQGAP1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with IQGAP1 Recombinant Rabbit Monoclonal Antibody (JG38-74) (Product # MA5-34789, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to IQGAP1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of IQGAP1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with IQGAP1 Recombinant Rabbit Monoclonal Antibody (JG38-74) (Product # MA5-34789) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with Hoechst 33342 (Product # H1399). F-actin (Panel c: Red) was stained with Alexa Fluor™ Plus 647 Phalloidin (Product # A30107, 1:2000 dilution). Panel d represents the merged image showing cytoplasm localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 40X magnification in CellInsight CX7 LZR High-Content Screening (HCS) Platform (Product # CX7A1110LZR) and externally deconvoluted (D.Sage et al. / Methods 115 (2017) 28–41).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of IQGAP1 in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IQGAP1 monoclonal antibody (Product # MA5-34789), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of IQGAP1 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IQGAP1 monoclonal antibody (Product # MA5-34789), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of IQGAP1 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IQGAP1 monoclonal antibody (Product # MA5-34789), followed by DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of IQGAP1 in paraffin-embedded mouse colon tissue. Samples were incubated with IQGAP1 monoclonal antibody (Product # MA5-34789), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of IQGAP1 in paraffin-embedded human spleen tissue. Samples were incubated with IQGAP1 monoclonal antibody (Product # MA5-34789), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of IQGAP1 in paraffin-embedded human placenta tissue. Samples were incubated with IQGAP1 monoclonal antibody (Product # MA5-34789), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of IQGAP1 in paraffin-embedded human lung cancer tissue. Samples were incubated with IQGAP1 monoclonal antibody (Product # MA5-34789), and followed by hematoxylin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of IQGAP1 in SiHa cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with IQGAP1 monoclonal antibody (Product # MA5-34789) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

MA5-34789