HPA014055

antibody from Atlas Antibodies

Targeting: IQGAP1 HUMORFA01, KIAA0051, p195, SAR1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA014055

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA014055, RRID:AB_1851856

Product name

Anti-IQGAP1

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

NRALESGDVNTVWKQLSSSVTGLTNIEEENCQRYL
DELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQ
EEHERILAIGLINEALDEGDAQKTLQALQIPAAKL
EGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWL
DEIQGG


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line A-431.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human endometrium and skeletal muscle tissues using Anti-IQGAP1 antibody. Corresponding IQGAP1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human epididymis shows moderate membranous positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN