PA5-95974

antibody from Invitrogen Antibodies

Targeting: SLC25A4 ANT1, PEO2, PEO3, T1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95974

Provider

Invitrogen Antibodies

Product name

ANT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: SW620, BT-474, OVCAR-3, HepG2, Mouse brain; Cellular Location: Mitochondrion inner membrane, Multi-pass membrane protein Immunogen sequence: VGKGAAQREF HGLGD

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.06 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using SLC25A4 Polyclonal antibody (Product # PA5-95974). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ANT in extracts of various cell lines using ANT Polyclonal Antibody (Product # PA5-95974) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ANT was performed in HeLa cells using ANT Polyclonal Antibody (Product # PA5-95974) at a dilution of 1:100. Blue: DAPI for nuclear staining.