Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF4627 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Collagen XIII alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Collagen XIII alpha 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human (rh) COL13A1v4 is observed, and less than 1% cross-reactivity with rhCOL4A1, rhCOL25A1, rhCOL3A1, and rhCOL1A1 is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Collagen XIII alpha 1 by Western Blot. Western blot shows lysates of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Collagen XIII alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4627) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Collagen XIII alpha 1 at approximately 85-95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Collagen XIII alpha 1 in Mouse Embryo. Collagen XIII alpha 1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Collagen XIII alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4627) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.