Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF6346 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Collagen XIII alpha 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Collagen XIII in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse COL13A1 and less than 1% cross-reactivity with recombinant human (rh) COL23A1 and rhCOL25A1 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_542992
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Membrane associated collagen XIII promotes cancer metastasis and enhances anoikis resistance.
Zhang H, Fredericks T, Xiong G, Qi Y, Rychahou PG, Li JD, Pihlajaniemi T, Xu W, Xu R
Breast cancer research : BCR 2018 Oct 1;20(1):116
Breast cancer research : BCR 2018 Oct 1;20(1):116
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human Collagen XIII alpha 1 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells. PVDF Membrane was probed with 1 µg/mL of Human Collagen XIII alpha 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6346) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Two specific bands were detected for Collagen XIII a1 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.