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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00010541-M05 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010541-M05, RRID:AB_10963243
- Product name
- ANP32B monoclonal antibody (M05), clone 8E10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant ANP32B.
- Antigen sequence
MDMKRRIHLELRNRTPAAVRELVLDNCKSNDGKIE
GLTAEFVNLEFLSLINVGLISVSNLPKLPKLKKLE
LSENRIFGGLDMLAEKLPNLTHLNLSGNKLKDIST
LEPLKKLECLKSLDLFNCEVTNLNDYRESVFKLLP
QLTYLDGYDREDQEAPDSDAEVDGVDEEEEDEEGE
DEEDEDDEDGEEEEFDEEDDEDEDVEGDEDDDEVS
EEEEEFGLDEEDEDEDEDEEEEEGGKGEKRKRETD
DEGEDD- Isotype
- IgG
- Antibody clone number
- 8E10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ANP32B expression in transfected 293T cell line by ANP32B monoclonal antibody (M05), clone 8E10.Lane 1: ANP32B transfected lysate (Predicted MW: 28.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ANP32B is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to ANP32B on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
