Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunohistochemistry [2]
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- Product number
- PA5-18144 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHAPI2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 16,000.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SSP29/ANP32B in Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35µg protein in RIPA buffer). Samples were probed with the SSP29/ANP32B antibody (Product # PA5-18144, 0.1µg/mL) for 1 hour. Western blot was detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SSP29/ANP32B in Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35µg protein in RIPA buffer). Samples were probed with the SSP29/ANP32B antibody (Product # PA5-18144, 0.1µg/mL) for 1 hour. Western blot was detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SSP29/ANP32B in Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35µg protein in RIPA buffer). Samples were probed with the SSP29/ANP32B antibody (Product # PA5-18144, 0.1µg/mL) for 1 hour. Western blot was detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHAPI2 by a PHAPI2 monoclonal antibody (Product # PA5-18144) at a concentration of 0.1 µg/mL. NIH3T3 cell lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PHAPI2 by a PHAPI2 monoclonal antibody (Product # PA5-18144) at a concentration of 0.1 µg/mL. Human Tonsil (A) and Rat (B) and Pig (C) Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of A431 cell lysate using Product # PA5-18144 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SSP29/ANP32B in paraffin embedded Human Breast tissue. Steamed antigen retrieval was performed with citrate buffer pH 6, followed by the application of the SSP29/ANP32B antibody (Product # PA5-18144) (3.8µg/mL) and AP-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PHAPI2 in paraffin embedded human breast using a PHAPI2 polyclonal antibody (Product #PA5-18144) at a concentration of 3.8 µg/mL. Steamed antigen retrieval was performed with pH 6 buffered citrate. Samples were then stained with alkaline phosphatase.