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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Product number
- PA1-815 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RXRB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-815 detects retinoid X receptor (RXR) beta from human and mouse cells as well as transfected human and mouse RXR beta. PA1-815 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~50 kDa protein representing RXR beta from COS cells transfected with the human gene. Gel shift assays reveal supershifting of vitamin D receptor (VDR)/RXR beta/vitamin D receptor response element (VDRE) complexes when PA1-815 is preincubated with RXR beta prior to addition of VDR. The PA1-815 immunogen is a synthetic peptide corresponding to residues Q(190) K S D Q G V E G P G A T(202) of mouse RXR beta. Recombinant human VDR (Cat. # RP-710) and human RXR beta (catalog # RP-812) proteins can be purchased separately for control experiments in Western blot, immunoprecipitation and gel shift procedures.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Thymocyte responsiveness to endogenous glucocorticoids is required for immunological fitness.
Thyroid hormone-mediated negative transcriptional regulation of Necdin expression.
The expression of retinoic acid receptor alpha is increased in the granule cells of the dentate gyrus in schizophrenia.
Retinoid x receptor agonists increase bcl2a1 expression and decrease apoptosis of naive T lymphocytes.
Induction of ovarian cancer cell apoptosis by 1,25-dihydroxyvitamin D3 through the down-regulation of telomerase.
Expression patterns of retinoid X receptors, retinaldehyde dehydrogenase, and peroxisome proliferator activated receptor gamma in bovine preattachment embryos.
Heterodimeric DNA binding by the vitamin D receptor and retinoid X receptors is enhanced by 1,25-dihydroxyvitamin D3 and inhibited by 9-cis-retinoic acid. Evidence for allosteric receptor interactions.
Heterodimeric DNA binding by the vitamin D receptor and retinoid X receptors is enhanced by 1,25-dihydroxyvitamin D3 and inhibited by 9-cis-retinoic acid. Evidence for allosteric receptor interactions.
Dominant negative retinoid X receptor beta inhibits retinoic acid-responsive gene regulation in embryonal carcinoma cells.
Retinoid X receptors stimulate and 9-cis retinoic acid inhibits 1,25-dihydroxyvitamin D3-activated expression of the rat osteocalcin gene.
Mittelstadt PR, Monteiro JP, Ashwell JD
The Journal of clinical investigation 2012 Jul;122(7):2384-94
The Journal of clinical investigation 2012 Jul;122(7):2384-94
Thyroid hormone-mediated negative transcriptional regulation of Necdin expression.
Nygård M, Becker N, Demeneix B, Pettersson K, Bondesson M
Journal of molecular endocrinology 2006 Jun;36(3):517-30
Journal of molecular endocrinology 2006 Jun;36(3):517-30
The expression of retinoic acid receptor alpha is increased in the granule cells of the dentate gyrus in schizophrenia.
Rioux L, Arnold SE
Psychiatry research 2005 Jan 30;133(1):13-21
Psychiatry research 2005 Jan 30;133(1):13-21
Retinoid x receptor agonists increase bcl2a1 expression and decrease apoptosis of naive T lymphocytes.
Rasooly R, Schuster GU, Gregg JP, Xiao JH, Chandraratna RA, Stephensen CB
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):7916-29
Journal of immunology (Baltimore, Md. : 1950) 2005 Dec 15;175(12):7916-29
Induction of ovarian cancer cell apoptosis by 1,25-dihydroxyvitamin D3 through the down-regulation of telomerase.
Jiang F, Bao J, Li P, Nicosia SV, Bai W
The Journal of biological chemistry 2004 Dec 17;279(51):53213-21
The Journal of biological chemistry 2004 Dec 17;279(51):53213-21
Expression patterns of retinoid X receptors, retinaldehyde dehydrogenase, and peroxisome proliferator activated receptor gamma in bovine preattachment embryos.
Mohan M, Malayer JR, Geisert RD, Morgan GL
Biology of reproduction 2002 Mar;66(3):692-700
Biology of reproduction 2002 Mar;66(3):692-700
Heterodimeric DNA binding by the vitamin D receptor and retinoid X receptors is enhanced by 1,25-dihydroxyvitamin D3 and inhibited by 9-cis-retinoic acid. Evidence for allosteric receptor interactions.
Thompson PD, Jurutka PW, Haussler CA, Whitfield GK, Haussler MR
The Journal of biological chemistry 1998 Apr 3;273(14):8483-91
The Journal of biological chemistry 1998 Apr 3;273(14):8483-91
Heterodimeric DNA binding by the vitamin D receptor and retinoid X receptors is enhanced by 1,25-dihydroxyvitamin D3 and inhibited by 9-cis-retinoic acid. Evidence for allosteric receptor interactions.
Thompson PD, Jurutka PW, Haussler CA, Whitfield GK, Haussler MR
The Journal of biological chemistry 1998 Apr 3;273(14):8483-91
The Journal of biological chemistry 1998 Apr 3;273(14):8483-91
Dominant negative retinoid X receptor beta inhibits retinoic acid-responsive gene regulation in embryonal carcinoma cells.
Minucci S, Zand DJ, Dey A, Marks MS, Nagata T, Grippo JF, Ozato K
Molecular and cellular biology 1994 Jan;14(1):360-72
Molecular and cellular biology 1994 Jan;14(1):360-72
Retinoid X receptors stimulate and 9-cis retinoic acid inhibits 1,25-dihydroxyvitamin D3-activated expression of the rat osteocalcin gene.
MacDonald PN, Dowd DR, Nakajima S, Galligan MA, Reeder MC, Haussler CA, Ozato K, Haussler MR
Molecular and cellular biology 1993 Sep;13(9):5907-17
Molecular and cellular biology 1993 Sep;13(9):5907-17
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