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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-07100 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-07100, RRID:AB_1625508
- Product name
- Mouse Monoclonal TMEFF2/Tomoregulin-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TMEFF2/Tomoregulin-2 Antibody (J4B6) [NBP1-07100] - The Cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TMEFF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: SW480 cell lysate
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TMEFF2/Tomoregulin-2 Antibody (J4B6) [NBP1-07100] - The Cell and Tissue lysates (30ug) were resolved by SDSPAGE, transferred to NC membrane and probed with antihuman TMEFF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TMEFF2/Tomoregulin-2 Antibody (J4B6) [NBP1-07100] - The Cell and Tissue lysates (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human TMEFF2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse brain tissue lysate Lane 2.: HeLa cell lysate
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: TMEFF2/Tomoregulin-2 Antibody (J4B6) [NBP1-07100] - ICC/IF analysis of TMEFF2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human TMEFF2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TMEFF2/Tomoregulin-2 Antibody (J4B6) [NBP1-07100] - Human TMEFF2 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)
