R35130

antibody from NSJ Bioreagents

Targeting: NKX2-5 CSX, CSX1, NKX2.5, NKX2E, NKX4-1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R35130

Provider

NSJ Bioreagents

Product name

NKX2.5 Antibody

Antibody type

Polyclonal

Description

This highly specific NKX2.5 antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Goat

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide

Storage

Aliquot and store the NKX2.5 antibody at -20oC.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human heart lysate with NKX2.5 antibody at 0.3ug/ml. Predicted molecular weight ~35kDa, routinely observed at 35~45kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse heart tissue lysate with NKX2.5 antibody at 1ug/ml. Predicted molecular weight ~35 kDa, routinely observed at 35~45 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human heart tissue with NKX2.5 antibody at 3.75ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human heart tissue with NKX2.5 antibody (left) and without NKX2.5 antibody (right). Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.