PA5-63387

antibody from Invitrogen Antibodies

Targeting: FKBP10 FKBP6, FKBP65, FLJ20683, FLJ22041, FLJ23833, hFKBP65

Western blot (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-63387

Provider

Invitrogen Antibodies

Product name

FKBP10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: GLPTGYLFVW HKDPPANLFE DMDLNKDGEV PPEEFSTFIK AQVSEGKGRL MPGQDPEKTI GDMFQNQDRN QDGKITV Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 88%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.2 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

FKBP10 Acts as a New Biomarker for Prognosis and Lymph Node Metastasis of Gastric Cancer by Bioinformatics Analysis and in Vitro Experiments.

Gong LB, Zhang C, Yu RX, Li C, Fan YB, Liu YP, Qu XJ
OncoTargets and therapy 2020;13:7399-7409

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 FKBP10 promotes adhesion of GC cells (A) FKBP10 protein expression in gastric cancer cell line and GES-1. ( B ) and ( C ) FKBP10 mRNA and protein expression levels were tested by qRT-PCR and Western blot analysis in HGC-27 and MKN-7 cells after transfected with siFKBP10 for 48 hours. ( D ) Adhesion assay was used to detect changes in adhesion ability of HGC-27 and MKN-7 cells after transfected with siFKBP10 for 48 hours. ( E ) Quantifications of cells are shown as proportions of the number of control cells. Original magnification, 200x. Scale bar: 100 uM. ( F ) and ( G ) Integrin alpha1, integrin alpha2, integrin alpha5, integrin alphaV, integrin alpha6, AKT, P-AKT 308, P-AKT 473 proteins were detected by Western blot analysis in HGC-27 and MKN-7 cells after transfected with siFKBP10 for 48 hours. beta-actin was used as a loading control in Western blot. (*P