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antibody from Invitrogen Antibodies
Targeting: FKBP10
FKBP6, FKBP65, FLJ20683, FLJ22041, FLJ23833, hFKBP65
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-63387 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FKBP10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GLPTGYLFVW HKDPPANLFE DMDLNKDGEV PPEEFSTFIK AQVSEGKGRL MPGQDPEKTI GDMFQNQDRN QDGKITV Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 88%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references FKBP10 Acts as a New Biomarker for Prognosis and Lymph Node Metastasis of Gastric Cancer by Bioinformatics Analysis and in Vitro Experiments.
Gong LB, Zhang C, Yu RX, Li C, Fan YB, Liu YP, Qu XJ
OncoTargets and therapy 2020;13:7399-7409
OncoTargets and therapy 2020;13:7399-7409
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FKBP10 in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG. Samples were probed using a FKBP10 Polyclonal Antibody (Product # PA5-63387).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FKBP10 in human endometrium and duodenum tissues using FKBP10 Polyclonal Antibody (Product # PA5-63387). Corresponding FKBP10 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FKBP10 in human endometrium using FKBP10 Polyclonal Antibody (Product # PA5-63387) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of FKBP10 in human duodenum using FKBP10 Polyclonal Antibody (Product # PA5-63387) shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 FKBP10 promotes adhesion of GC cells (A) FKBP10 protein expression in gastric cancer cell line and GES-1. ( B ) and ( C ) FKBP10 mRNA and protein expression levels were tested by qRT-PCR and Western blot analysis in HGC-27 and MKN-7 cells after transfected with siFKBP10 for 48 hours. ( D ) Adhesion assay was used to detect changes in adhesion ability of HGC-27 and MKN-7 cells after transfected with siFKBP10 for 48 hours. ( E ) Quantifications of cells are shown as proportions of the number of control cells. Original magnification, 200x. Scale bar: 100 uM. ( F ) and ( G ) Integrin alpha1, integrin alpha2, integrin alpha5, integrin alphaV, integrin alpha6, AKT, P-AKT 308, P-AKT 473 proteins were detected by Western blot analysis in HGC-27 and MKN-7 cells after transfected with siFKBP10 for 48 hours. beta-actin was used as a loading control in Western blot. (*P
