Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-30648 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Macro H2A.2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: mouse brain, human H2AFY2-transfected 293T. Predicted reactivity: Mouse (99%), Rat (99%), Xenopus laevis (84%), Chicken (95%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Macro H2A.2 Polyclonal Antibody (Product # PA5-30648). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. Macro H2A.2 Polyclonal Antibody (Product # PA5-30648) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Macro H2A.2 antibody detects Macro H2A.2 protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg whole cell lysate/extract of human H2AFY2-transfected 293T cells.10 % SDS-PAGE. Macro H2A.2 antibody Macro H2A.2 Polyclonal Antibody (Product # PA5-30648) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Macro H2A.2 in paraformaldehyde-fixed A431 cells using a Macro H2A.2 polyclonal antibody (Product # PA5-30648) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Macro H2A.2 was performed in paraffin-embedded mouse brain tissue using Macro H2A.2 Polyclonal Antibody (Product # PA5-30648) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using Macro H2A.2 (Product # PA5-30648) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.