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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-90703 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AVIL Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: AAVQVRVRMG TEPRHFMAIF KGKLVIFEGG TSRKGNAEPD PPVRLFQIHG NDKSNTKAVE VPAFASSLNS NDVFLLRTQA EHYLWYGKGS SGDERAMAKE LASLLCDGSE NTVAEGQEPA EFWDLLGGKT PYANDKRLQQ EILDVQSRLF ECSNKTGQFV VTEITDFTQD DLNPTDVMLL DTWDQVFLWI GAEANATEKE SALATAQQYL HTHPSGRDPD TPILIIKQGF EPPIFTGWFL AWDPNIWSAG KTYEQLKEEL GDAAAIMRIT ADMKNATLSL NSNDSEPKYY PIAVLLKNQN QELPEDVNPA KKENYLSEQD FVSVFGITRG QFAALPGWKQ LQMKKEKGLF; Positive Samples: HT-29, Mouse uterus, Mouse brain, Rat uterus, Rat testis, Rat brain; Cellular Location: Cell projection, Cytoplasm, axon, cytoskeleton
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.23 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using AVIL Polyclonal antibody (Product # PA5-90703) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AVIL in extracts of various cell lines using AVIL Polyclonal Antibody (Product # PA5-90703) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of AVIL was performed in L929 cells using AVIL Polyclonal Antibody (Product # PA5-90703) at a dilution of 1:100. Blue: DAPI for nuclear staining.
