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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [2]
- Other assay [3]
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- Product number
- PA5-117800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ATL3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of ATL3 in Lane A: Mouse liver Tissue lysate, Lane B: 293T Whole Cell lysate, Lane C: NIH-3T3 Whole Cell lysate, Lane D: Jurkat Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117800) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG H&L (Dylight 800) using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 61 kDa, Observed band size: 61 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ATL3 Polyclonal Antibody (Product # PA5-117800) at 1:500 dilution. Lane A: Mouse liver Tissue Lysate, Lane B: 293T Whole Cell Lysate, Lane C: NIH-3T3 Whole Cell Lysate, Lane D: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa. (We are unsure of the identity of these extra bands).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATL3 in Lane A: 0.5 mg Jurkat Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-117800) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 61 kDa, Observed band size: 61 kDa .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATL3 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 µL with ATL3 Polyclonal Antibody (Product # PA5-117800) and 15 µL of 50 % Protein G agarose. Primary antibody: ATL3 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATL3 in Lane A: 0.5 mg Jurkat Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-117800) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 61 kDa, Observed band size: 61 kDa .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ATL3 in Lane A: 0.5 mg Jurkat Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-117800) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 61 kDa, Observed band size: 61 kDa .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ATL3 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 µL with ATL3 Polyclonal Antibody (Product # PA5-117800) and 15 µL of 50 % Protein G agarose. Primary antibody: ATL3 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa.
