PA5-117800

antibody from Invitrogen Antibodies

Targeting: ATL3 DKFZP564J0863

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117800

Provider

Invitrogen Antibodies

Product name

ATL3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of ATL3 in Lane A: 0.5 mg Jurkat Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-117800) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 61 kDa, Observed band size: 61 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of ATL3 in Lane A: 0.5 mg Jurkat Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-117800) with a dilution of 1:100 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:1,000. Assay was performed under reducing conditions. Predicted band size: 61 kDa, Observed band size: 61 kDa .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ATL3 Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 2 µL with ATL3 Polyclonal Antibody (Product # PA5-117800) and 15 µL of 50 % Protein G agarose. Primary antibody: ATL3 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1,000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 61 kDa. Observed band size: 61 kDa.