F54565

antibody from NSJ Bioreagents

Targeting: VPS52 ARE1, SACM2L

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54565

Provider

NSJ Bioreagents

Product name

VPS52 Antibody / SACM2L

Antibody type

Polyclonal

Description

This highly specific VPS52 antibody is suitable for use in Immunofluorescence/Flow cytometry/Immunohistochemistry/Western blot applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the VPS52 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human MDA-MB-435 cell lysate with VPS52 antibody. Predicted molecular weight ~82 kDa (isoform 1) and 68 kDa (isoform 2).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of human MCF7 cells with VPS52 antibody (green) and DAPI nuclear stain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human colon carcinoma tissue with VPS52 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MDA-MB-435 cells with VPS52 antibody; Blue=isotype control, Green= VPS52 antibody.